Scrambled Peptide Library Construction


A Scrambled Peptide Library is used for finding new leads by creating a random screening library. ALL Chemistry have the capability to construct any Scrambled Peptide Library at your request. Our scientists have accumulated extensive experience in different types of Peptide Library Construction. 


Introduction of Scrambled Peptide Library


The design of Scrambled Library is based on the internal substitution of the original polypeptide amino acid sequence. It is the most mutated polypeptide library, and the disrupted polypeptide is usually used as a negative control to confirm that a specific sequence is the key to the function or activity of the protein. It is also a random screening tool to discover new targets. The construction of the Scrambled Peptide Library is obtained by generating a complete rearrangement of the original polypeptide sequence. Its amino acid composition is the same as that of the original peptide, but the sequence is different, so that all possible alternative choices can be obtained and the polypeptide library with the highest mutation degree can be provided.

The scrambled library is constructed on the basis of changing the sequence of the original peptide. The number of each amino acid in the peptide (e.g. 2 arginines) is the same, but its position with the rest of the amino acids is random. The scrambled library has the highest variation of any peptide library, displaying all possible peptide substitution structures and it is an ideal sequence optimization method. The purpose of this library is to initially identify peptides that have specific functions or activities among a set of active sequences. A scrambled library is a tool for finding new leads by creating a random screening library. They are usually used as targeted molecular probes for proteins, antibodies, genes, etc.


Fig.1 Scrambled Library


Applications of Scrambled Peptide Library


--Peptide Sequence Optimization: preliminary identification of peptides that may have specific functions or activities in a group of active sequences. Scrambled Peptide Library plays a pivotal role in peptide sequence optimization. By generating randomized peptide sequences, researchers can explore a vast sequence space and identify peptides with improved properties such as enhanced binding affinity, stability, and selectivity. Through screening techniques like phage display or affinity chromatography, peptides with desired characteristics can be selected and further optimized.

--Objective Protein Screening: This approach allows for the discovery of novel protein-protein interactions, identification of binding motifs, and exploration of protein function. Scrambled Peptide Library serves as a valuable resource for unbiased screening, enabling the identification of peptides that may not have been predicted based on known protein-binding motifs.

--Negative Control: the resulting peptides are often used as negative controls to show that specific sequences rather than amino acid composition are critical to activity. Scrambled Peptide Library can be used as a negative control, generating peptides with randomized sequences that lack specific binding affinity. This control is crucial in distinguishing specific interactions from non-specific ones, ensuring the reliability and validity of experimental findings.

--Targeted Molecular Probes: Targeted molecular probes  for proteins, antibodies, genes, etc, by screening the library against specific proteins, antibodies, or genes, researchers can identify peptides that bind with high affinity and specificity. These peptides can be further modified with fluorescent or radioactive tags, enabling their use as molecular probes for imaging, quantification, or isolation of specific targets.



Fig.2 Original Sequence


Scrambled Peptide Library Services at ALL Chemistry


ALL Chemistry provides high-quality scrambled library design and construction services, supported by our scientists' accumulated knowledge and experience in carbohydrate chemistry. We have been designing, synthesizing and applying peptide library technology for many years. According to strict quality assurance and biosynthetic quality control standards, the peptide is strictly monitored during the production cycle. We provide consultations for creating the most efficient and economical library design and help you compute the list of peptide library sequences. Please feel free to contact our experts to design your next successful peptide library.


Advantages of Our Services


Low Cost, Rapidity and Easy Operability.

Accuracy, Visibility and Easy Analysis.

Competitive Prices: Our peptide library services are the most cost-efficient on the market.

Flexible Purity Choices: Crude, desalt, >70%, >75%, >80%, 85%, >90%, >95%, and >98% purity are available to meet your multiple demands.

No Cross-contamination: Peptides are supplied in individual well-labeled vials.

Comprehensive Modifications: Our modification service includes labeling, the incorporation of unnatural amino acids, and peptide cyclization with disulfide bridges.

Stringent Quality Control: ALL Chemistry provides MS and HPLC validation data for each peptide.

Chemistry provides epitope mapping service, binding assay, and functional assay services for your drug discovery research.


Project Workflow


Evaluation → Experiment Design → Formal Quotation → Scrambled Peptide Library Construction → Results → Delivery