Alanine Peptide Scanning Library Construction


ALL Chemistry is a professional supplier that provides customizable, fully synthetic peptide libraries for mid- to high-throughput applications. As an important tool for peptide sequence optimization important tool for peptide sequence optimization, functional peptide scanning library is recommended for studying the fuction of the required library.


Introduction of Alanine Peptide Scanning Library


Alanine Scanning can be used as a tool to identify specific amino acid residues that are responsible for the peptide's function, stability, and conformation. Alanine, the smallest chiral amino acid, is sequentially substituted for each non-alanine residue at a time. The small methyl side chain of the alanine residue has relatively little biological activity, therefore, a decrease in epitope activity is observed when an essential amino acid is replaced. In instances the size preservation is important, the amino acid may be replaced by a valine or leucine residue instead of an alanine residue. Substitution of key amino acid residue(s) with alanine causes changes in epitope binding activity. This library enables quick determination of each individual amino acid's contribution to the peptide's functionality.

The Alanine Peptide Scanning Library is particularly useful in the design and optimization of peptide-based therapeutics, where understanding the key amino acids involved in protein recognition and binding is crucial. By systematically screening the library against a target protein or receptor, researchers can identify the specific residues that contribute significantly to binding affinity and selectivity. This information can then be utilized to engineer more potent and selective peptides for therapeutic applications. The Alanine Peptide Scanning Library offers a valuable platform for investigating the structure-activity relationship of peptides and understanding the molecular basis of peptide-protein interactions. Its applications in drug discovery, protein engineering, and peptide-based therapeutics make it an indispensable tool in the field of peptide chemistry.



Fig.1 Original Sequence

Applications of Alanine Peptide Scanning Library 


The applications of alanine scanning libraries include: identifying key residues in epitopes, evaluating the functional properties of enzymes, and studying binding sites of proteins.

--Identify Key Residues in Epitopes: Epitopes are specific regions on antigens that are recognized and bound by antibodies or T-cell receptors. Understanding the key residues within an epitope is crucial for developing vaccines, diagnostic tools, and therapeutic antibodies. The Alanine Peptide Scanning Library can help identify these critical amino acid residues. By systematically substituting each residue in the epitope peptide with alanine and assessing its binding affinity with the antibody or receptor, researchers can pinpoint the residues essential for the interaction. This information can be used to design epitope-based vaccines or develop antibodies with improved specificity and affinity.        

--Evaluation Function Property of Enzymes: Enzymes play a vital role in catalyzing biochemical reactions, and understanding their functional properties is essential for various applications, including biotechnology and drug discovery. The Alanine Peptide Scanning Library can be used to evaluate the contribution of each amino acid residue to enzyme activity. By systematically replacing each residue in the enzyme peptide with alanine and measuring the enzymatic activity, researchers can identify key residues critical for the enzyme's function. This information can help in enzyme engineering, where specific residues can be modified to enhance catalytic efficiency or alter substrate specificity.

--Study Binding Sites of Proteins: Protein-ligand interactions are fundamental in various biological processes, including drug-target interactions and signal transduction pathways. Understanding the binding sites within proteins is crucial for designing small molecule inhibitors or optimizing drug candidates. The Alanine Peptide Scanning Library can aid in studying these binding sites. By systematically replacing each residue in the protein sequence with alanine and assessing the impact on ligand binding or protein function, researchers can identify critical amino acid residues involved in the interaction. This information can guide the rational design of small molecules or peptides that target these binding sites, leading to the development of more effective drugs.


Fig.2 Strategy for synthesizing a positional-scanning combinatorial substrate library for caspases


Alanine Peptide Scanning Library Services at ALL Chemistry

Alanine scanning library is one of the peptide library design tools. Alanine Scanning Library, this tool allows you to systematically substitute alanine for each amino acid position in your peptide sequence. Alanine is the smallest of the naturally occurring amino acids. It can maintain its chirality. In molecular biology, alanine scanning is a site-directed mutagenesis technique used to determine the contribution of specific residues to the stability or function of a given protein. Alanine scanning library can be a powerful tool to explore the effects of single amino acid side chains on protein functions and properties. Alanine can be substituted for each non-alanine residue in turn, one at a time, and subsequently, the corresponding change in epitope activity can be measured. Substituting alanine for key amino acid residues will cause changes in epitope binding activity, so that the contribution of each amino acid to peptide function can be quickly determined. ALL Chemistry is capable of producing a high-quality alanine peptide scanning library for scientists all over the world. 

Example: Peptide Affinity Maturation by Alanine Peptide Scanning

Alanine scanning mutagenesis is our preferred method for the affinity maturation of peptide binders. In this method, every single amino acid of the selected binding peptide will be replaced with an alanine, and then the binding of the modified peptides to the target protein will be assayed using BIAcore technology. The non-essential amino acid will be specifically identified. Then, we will create a directed/constrained peptide sub-library containing random sequences on the non-essential amino acid positions. Mutants with increased binding affinity are identified by enhancing the screening stringency, followed by phage ELISA. 


Advantages of Our Services


ALL Chemistry guarantees that the purity of the crude peptide library is > 30%. The purity of the crude product can also be as high as 80%, which mainly depends on the characteristics of the polypeptide sequence itself.

Pure peptide library, according to customer needs, we can provide different levels of purity: > 70%, > 80, or even 98%.

The length of each polypeptide is 5-20aa.

Polypeptide modification includes fluorescence labeling, biotin labeling and D- amino acids.

The minimum starting quantity of polypeptide library is 48 peptides.

The general delivery time is 2-3 weeks. The peptide library was directly freeze-dried in a 96-well plate, which can be directly used in subsequent experiments.

Strict Quality Control: Each polypeptide product is provided with MS and HPLC identification results.


Project Workflow


Evaluation → Experiment Design → Formal Quotation → Peptide Scanning Library Construction → Results → Delivery