Custom cDNA Cloning


cDNA cloning is a molecular biology technique used to generate a DNA copy of an RNA molecule. This technique is used to study gene expression and identify specific RNA molecules that are present in a sample. The process involves using reverse transcriptase to synthesize a complementary DNA (cDNA) strand from the RNA template. The cDNA can then be amplified using polymerase chain reaction (PCR) and cloned into a vector for further analysis. cDNA cloning has many applications, including the study of gene expression, the identification of disease-associated genes, and the production of recombinant proteins for use in biotechnology.

ALL Chemistry Inc. offers custom cDNA cloning services that involve the production of a custom cDNA library from a specific tissue or cell type. Our Custom cDNA cloning services can be tailored to meet the specific needs of a research project or application. Some of the factors that can be customized include the tissue or cell type used for the cDNA library, the vector and cloning method used, and the sequencing technology used for analysis. These services can be used in a wide range of applications, including drug discovery, gene expression analysis, and biotechnology research.


Our Advantages:


Different Vector Expression Systems Available (Mammalian expression, Lentiviral system, AAV, Bacterial)

Multiple fluorescent and epitope tag versions of vectors for easy analysis and visualization of protein expression levels

The full length open reading frame or specific domains or generate specific mutations

Rapid Turnaround Time


Different tags/selection markers:


Epitope tags (for easy protein purification and detection): Flag/DDK, HA, His, Myc, FC, GST, hFc, mFc

Fluorescent tags (for easy protein tracking and visualization): GFP, RFP, BFP, YFP, Cyan, Far-red

Mammalian selection markers: neomycin, puromycin, blasticidin, Hygromycin

Different promoters: CMV, EF1a


ALL Chemistry Inc tailors the cDNA preparation process to meet your requirements. cDNA concentration, amount, buffer, and lyophilization can all be adjusted to meet your specifications. Feel free to contact us for more information.

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