ALL Chemistry can construct any Truncation Peptide Library at your request. Our scientists have accumulated extensive experience in different types of Peptide Library Construction. All our efforts are focused on meeting customers' specific demands.
Introduction of Truncation Peptide Library
Truncation peptide library can serve as a tool to investigate the extent to which peptide small molecules undergo metabolic degradation, which is a major consideration in bringing small molecules to the market. Truncation Peptide Library is used to determine the minimum amino acid length required for optimal epitope activity, and the method of establishing it is to systematically remove the amino acids at both ends of the original polypeptide to obtain some series of polypeptides. These peptides are then tested against the same target as the parent peptide. The main advantage of this library is the reduction in the cost of mass production. For instance, Jones, Turpin, Bultmann, Brandt, and Schultz-Cherry (2006) identified a minimal sequence of the entry blocker possessing antiviral activity against the replication of influenza A virus. They serially deleted residues from both termini and concluded that the peptide B10NP with 10 amino acids was able to inhibit the virus with comparable antiviral activity as the 20 amino acid long parent peptide.
If some important amino acids are known, we should keep these amino acid sequences and then remove them one by one from the other end of the peptide to start screening. The original peptide sequence is systematically truncated by different monomeric units, and each peptide produced by this method can then be tested. Long peptides can be used in conjunction with alanine scanning libraries. Truncation library screening allows the identification of peptides with enhanced proteolytic stability. Another example is the identification of an anti-HBV peptide from pre-S1 protein by truncation of peptide pre-S1. The peptide identified was further truncated and resulted in pre-S1 Myr peptide which was found to inhibit the HBV replication at nM (nanomolar) concentrations.
Fig.1 Schematic presentation of constructing the CX7C-peptide library in phage and the principle of in vivo phage display.
Applications of Truncation Peptide Library
Truncation peptide libraries have become increasingly popular due to their ability to identify the best epitope sequence and the optimal binding site of localization proteins.
--Identify the Best Epitope Sequence: Epitopes are specific regions on proteins that are recognized and bound by antibodies or other proteins. Identifying the best epitope sequence is crucial for various applications, such as vaccine development, antibody production, and targeted drug delivery. By generating a truncation peptide library, researchers can create a collection of overlapping peptides that cover the entire protein sequence. These peptides are then screened against the antibody or protein of interest to identify the specific sequence that exhibits the highest binding affinity.
--Optimal Binding Site of Localization Protein: Truncation peptide libraries can be utilized to identify the optimal binding site of a localization protein on its target protein. By systematically truncating the target protein sequence and screening the resulting peptides against the localization protein, researchers can pinpoint the specific region responsible for the interaction. This knowledge can aid in designing small molecules or peptides that can disrupt or enhance protein localization, thereby influencing cellular processes. They can also be used to study protein-protein interactions, map protein domains, and investigate the effects of post-translational modifications on protein function.
Truncation Peptide Library Services at ALL Chemistry
Truncation peptide library is generally used in combination with the alanine screening peptide library. After alanine is used to determine the key residues of the epitope, truncated peptide library is used to further determine the shortest epitope sequence and its function. In many cases, truncated peptide libraries are used to identify and screen peptides that enhance proteolysis. It can be used as a tool to study the metabolic degradation degree of polypeptide drugs. Scientists at ALL Chemistry continuously strive to produce high-quality Truncation Peptide Library Construction and related services to solve problems in scientific discovery.
Advantages of Our Services
Low Cost, Rapidity and Easy Operability.
Accuracy, Visibility and Easy Analysis.
Competitive Prices: Our peptide library services are the most cost-efficient on the market.
Flexible Purity Choices: Crude, desalt, >70%, >75%, >80%, 85%, >90%, >95%, and >98% purity are available to meet your multiple demands.
No Cross-contamination: Peptides are supplied in individual well-labeled vials.
Comprehensive Modifications: Our modification service includes labeling, the incorporation of unnatural amino acids, and peptide cyclization with disulfide bridges.
Stringent Quality Control: ALL Chemistry provides MS and HPLC validation data for each peptide.
Chemistry provides epitope mapping service, binding assay, and functional assay services for your drug discovery research.
Project Workflow
Evaluation → Experiment Design → Formal Quotation → Truncation Peptide Library Construction → Results → Delivery
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