ALL Chemistry can offer you high-quality peptide array services including peptide synthesis, modifications, peptide matrix for scientific research and small molecule screening.
Custom Acetylation & Deacetylation Peptide Array Service
Protein acetylation is one of the major post-translational modifications (PTMs) in eukaryotes, in which the acetyl group from acetyl coenzyme A (Ac-CoA) is transferred to a specific site on a polypeptide chain. Two types of acetylation are known to occur widely in proteins: α-amino group of the N-terminus and ε-amino group of lysine residues. Figure 1 shows the schematic overview of acetylation and deacetylation in proteins, such as histone and lysine residue.
Fig.1 The schematic overview of acetylation and deacetylation: A. Acetylation of α-amino group of the N-terminus, catalyzed by N-terminal acetyltransferases (NATs), irreversible. B. Acetylation or deacetylation of ε-amino group of a lysine residue, catalyzed by lysine
acetyltransferases (KATs) or Lysine deacetylases (KDACs), reversible.
Acetylation and deacetylation of proteins play a crucial function in the processes of cellular metabolism. To efficiently complete a high throughput screening for the substrates of acetylase or deacetylase of interest, ALL Chemistry carefully chooses the peptide substrates labeled/non-labeled by acetyl group in special sites (N-terminal and Lysine) from our database of acetylation sites and custom acetylation or deacetylation peptide array for our clients. Figure 2 shows a model of the peptide substrates array for acetylase or deacetylase: A. lysine acetylation and acetylation peptide array service; B. α-amino group of the N-terminus.
Service Specification
-- Lyophilized peptides, 5-250 nmole in each well of a 96-well plate
-- No cross contamination
-- Quality Control: MALDI-TOF QC and COA
-- Turnaround time: Typically, 2-3 week
Applications of Acetylation & Deacetylation Peptide Array
-- Acetylation and Deacetylation Profiling
-- Identification of kinases, using pre-defined substrates
-- Characterization of substrate specificity of a given acetylase or deacetylase
-- Comparison between/among multiple acetylases or deacetylases
-- Fingerprinting among/between different cells, tissues
At ALL Chemistry, we have already collected thousands of protein entries with >24,000 lysine acetylation sites, allowing us to create a unique solution for each project. Please contact us for more information and acetylation sites.
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